(-)-Epigallocatechin Gallate Reduces Platelet-Derived Growth Factor-BB-Stimulated Interleukin-6 Synthesis in Osteoblasts: Suppression of SAPK/JNK
Figure 3
Effect of EGCG on the phosphorylation of
p44/p42 MAP kinase and p38 MAP kinase induced by PDGF-BB in MC3T3-E1 cells. The
cultured cells were pretreated with the indicated doses of EGCG or vehicle for
60 minutes, and then stimulated by 50 ng/mL PDGF-BB or vehicle for 3 minutes. The
extracts of cells were subjected to SDS-PAGE with subsequent Western blotting
analysis with antibodies against (a) phospho-specific p44/p42 MAP kinase or
p44/p42 MAP kinase, and (b) phospho-specific p38 MAP kinase or p38 MAP kinase.
The histogram shows quantitative representations of the levels of
PDGF-BB-induced phosphorylation obtained from laser densitometric analysis of
three independent experiments. Each value represents the meanSEM of
triplicate determinations. Similar results were obtained with two additional
and different cell preparations.
