Toll-Like Receptors and Cytokines as Surrogate Biomarkers for Evaluating Vaginal Immune Response following Microbicide Administration

Table 1

Oligonucleotide primers used for the study.

Gene	Accession No	Primer sequence (5^′→3^′)	Product size (bp)	Annealing temperature
IL-4	DQ_852343	F-AGTTCTACCTCCACCACAAGGT	212	60^∘C
		R-TCAGCTCTGACGCTTTGAGTAT
IL-6	NM_001082064	F-AGGAGCTGAGGAAAGAGATGTG	225	58^∘C
		R-TGTTTTCTTCGTCACTCCTGAA
IL-10	NM_001082045	F-TTCTTTCAATCGAAGGATCAGC	267	59^∘C
		R-CTCATTCATGGCTTTGTAGACG
TNF-𝛼	ID_AB128153	F-CTAGCCCACGTAGTAGCAAACC	159	60^∘C
		R-GCTGAAGAGAACCTGGGAGTAG
TLR-3	NM_003265	F-GAT CTG TCT CAT AAT GGC TTG	305	60^∘C
		R-GAC AGA TTC CGA ATG CTT GTG
TLR-9	NM_017442	F-TTC CCT GTA GCT GCT GTC C	207	59^∘C
		R-ACA GCC AGT TGC AGT TCA CC
Defensin-1	NM_001082299	F-CTCTGCTTGCTGCCATTCTC	196	60^∘C
		R-AATCGTCTGCAAGTACAGGACAC
GAPDH	NM_01082253	F-CCATTCATTGACCTCCACTACA	199	59^∘C
		R-CGTTGCTGACAATCTTGAGAGA