Cardiotrophin-1 Induces Tumor Necrosis Factor Synthesis in Human Peripheral Blood Mononuclear Cells
Figure 1
(a) Concentration- and time-dependent expression of α protein in the supernatant after incubation with CT-1. Human PBMCs were incubated with various concentrations of CT-1 and for different periodes. After the indicated time α protein concentration was determined by a commercial available ELISA. , data are expressed as meanSEM. compared to unstimulated cells. (b) Analysis of intracellular α production using immunofluorescent flow cytometry. Human blood was incubated with different concentrations of CT-1 for 6 hours in the presence of brefeldin A. Afterwards erythrocytes were lysed and cells were stained with a monoclonal antibody against CD14 FITC-conjugated and against α PE-conjugated. Monocytes were gated and results are expressed normalized to unstimlulated monocytes. , data are expressed as meanSEM. compared to unstimulated cells.
