Inhibitory Effects of Ketamine on Lipopolysaccharide-Induced Microglial Activation

Figure 2

Tetrazolium dye 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay of ketamine-treated microglia. Microglia (5×10⁵ cells mL⁻¹) were treated with various concentrations of ketamine (100 ~ 500 μM) or an isovolumetric PBS buffer for 24 hours. Cell viability was measured by a colorimetric assay at 550 nm based on the ability of mitochondria to reduce the MTT in viable cells. Data are presented as the means ± S.E.M. (𝑛=3).