Maintained Smoking Cessation for 6 Months Equilibrates the Percentage of Sputum CD𝟖⁺ Lymphocyte Cells with That of Nonsmokers

Figure 2

Example of a flow cytometric analysis with dot plots and histograms from a representative sample (from one subject). First, epithelial cells were excluded from the analysis by gating on all CD45⁺ Fleukocytes (plot (a)). Then lymphocytes were gated by morphology on an FS/SS plot (plot (b)). Finally, CD8⁺ and CD4⁺ lymphocytes were gated (plot (c)). Staining of isotype control antibodies is also shown (plot (d)). PE: phycoerythrin, PC-5: phycoerythrin-cyanine, FITC: fluorescein isothiocyanate.

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