Chemokine Receptor and Ligand Upregulation in the Diaphragm
during Endotoxemia and Pseudomonas Lung Infection
Figure 1
CC-class chemokine receptor gene expression in the diaphragm and limb muscle at baseline and during endotoxemia in vivo. (a) Representative autoradiograph of ribonuclease protection assay data, showing CCR1 gene expression in the mouse diaphragm and tibialis anterior (TA) muscles at 24 hours following injection of either LPS or saline. (b) Quantification of CCR1 and CCR5 mRNA levels at 6 and 24 hours after LPS administration. Note that the samples for each time point were loaded onto separate gels, such that the absolute arbitrary units depicted are valid for quantitative comparisons within but not between time points. All data are group means ± SE, mice per group. Data are normalized to the L32 housekeeping gene. for comparisons between diaphragm and TA within the same condition (LPS or sham control); for comparisons between LPS- and sham-control- (saline-) treated mice within the same type of muscle (diaphragm or TA).
