Chemokine Receptor and Ligand Upregulation in the Diaphragm
during Endotoxemia and Pseudomonas Lung Infection
Figure 3
Bacterial dose- and time-dependence of chemokine gene expression in the diaphragm during Pseudomonas lung infection. (a) Representative ribonuclease protection assay data indicating RANTES and MIP-1α mRNA levels in the diaphragm at two separate time points (Day 2 and Day 7) following intratracheal instillation of Pseudomonas aeruginosa bacteria into the lungs at different doses ( or cfu). (b) Quantification of RANTES and MIP-1α gene expression under the above conditions. Note that only the higher inoculating dose of bacteria led to measurable chemokine upregulation at Day 2, while the lower dose was also able to induce a significant increase in RANTES gene expression by Day 7 postinfection. All data are group means ± SE, mice per group. Data are normalized to the L32 housekeeping gene. for comparisons to the sham control (CTL) condition. ND = signal not detectable.
