Chemokine Receptor and Ligand Upregulation in the Diaphragm
during Endotoxemia and Pseudomonas Lung Infection
Figure 5
LPS-induced activation of RANTES is abrogated by pharmacological inhibition of NF-κB but not reactive oxygen species. (a) The NF-κB inhibitor, PDTC, dramatically suppressed RANTES gene expression in primary diaphragmatic myotubes exposed to LPS. (b) In contrast, two classical antioxidant compounds, N-acetylcysteine (NAC) and catalase, had no measurable effect on RANTES gene expression by diaphragmatic myotubes exposed to LPS under the same conditions. The primary myotube cultures were pretreated with PDTC or the antioxidant compounds for one hour prior to initiating LPS stimulation for 4 hours (1 g/mL).
