Chemokine Receptor and Ligand Upregulation in the Diaphragm
during Endotoxemia and Pseudomonas Lung Infection
Figure 6
Effects of dominant negative inhibition of the NF-κB pathway on LPS-induced RANTES gene expression. (a) Ribonuclease protection assay showing the effects of transfecting expression plasmids encoding dominant negative constructs for IKKα and IKKβ, on RANTES expression by C2C12 myotubes exposed to LPS. Control cells were transfected in an identical fashion with a control plasmid (CTL-GFP) to determine transfection efficiency. (b) Quantification of RANTES mRNA levels in LPS-stimulated myotubes previously transfected with IKKα or IKKβ dominant negative mutant constructs. All data are normalized to the L32 housekeeping gene. for comparisons to the CTL-GFP group.
