Oxidative Damage in Lymphocytes of Copper Smelter Workers Correlated to Higher Levels of Excreted Arsenic

Figure 5

Effect of sodium arsenite on Jurkat cells. (a) Inhibition of proliferation of PHA-stimulated Jurkat cells by sodium arsenite. Cells (2×10⁵ cells/well) were incubated as indicated before, and proliferation was assessed by [³H]-TdR incorporation. Results are expressed in counts per minute (cpm) in the absence and presence of different sodium arsenite concentrations. (b) Evaluation of proliferation of cells (2×10⁵ cells/well) stimulated with 5 μg/ml PHA for 48 h. Results are expressed as the number of cells obtained in the presence of different sodium arsenite concentrations in the culture media. (c) Lipid peroxidation in Jurkat cells induced by arsenic. Cells (2×10⁵ cells/well) were incubated with sodium arsenite for 48 h and the level of lipid peroxides was measured in the homogenates as MDA concentration.

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