Regulatory Effect of Cinnamaldehyde on Monocyte/Macrophage-Mediated Inflammatory Responses

Figure 6

Effect of CA on cell-cell adhesion and cell-fibronectin adhesion. U937 cells pretreated with CA for 1 h were incubated in the presence or absence of anti-CD29 antibody MEM101A (1 $\mu$g/ml) or anti-CD43 antibody 161-46 (1 $\mu$g/ml). Images (a) of the cells in culture were obtained using an inverted phase contrast microscope attached to a video camera. Quantitative analysis (b) of cell-cell clusters was assessed using a quantitative cell-cell adhesion assay. (c) U937 cells pretreated with CA, P5D2, or U0126 for 30 min were seeded on fibronectin-coated (50 $\mu$g/ml) plates and further incubated for 3 h. The number of attached cells was determined by crystal violet assay. (d) U937 cells ($1\times {10}^6$cells/ml) were incubated with CA for 12 h. Surface levels of CD29, CD18, and CD43 were determined by flow cytometric analysis. (e) RAW264.7 cells ($2\times {10}^6$ cells/ml) pretreated with CA for 1 h were incubated for 48 h after scratching with a pipette. Images of the cells in culture were obtained using an inverted phase contrast microscope attached to a video camera. $\mathrm{\hspace{0.17em}\hspace{0.17em}}{}_{}{}^{*}P<.05$ and ${}_{}{}^{**}P<.01$ represent significant difference compared to control.

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