HPV16E6-Dependent c-Fos Expression Contributes to AP-1 Complex Formation in SiHa Cells

Figure 1

(a) Upregulation of AP-1 gene expression in SiHa cells at the indicated time points after H₂O₂ (1 mM) exposure. Representative blots are shown and include β-actin or tubulin as a loading control, along with the results of densitometric analysis (0 h, 4 h; normalized to β-actin or tubulin). *𝑃<0.05, 𝑛=3. (b) Inhibitory effect of o-phenanthroline on H₂O₂-induced c-fos upregulation. Cells were pretreated for 1 hour with or without o-phenanthroline (200 μM) before H₂O₂ (1 mM) exposure. Representative blots are shown and include β-actin as a loading control, along with the results of densitometric analysis (4 h; normalized to β-actin). *𝑃<0.05, 𝑛=4.

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