Tropism, Cytotoxicity, and Inflammatory Properties of Two Envelope Genes of Murine Leukemia Virus Type-Endogenous Retroviruses of C57BL/6J Mice
Figure 2
Coding potential and membrane localization of ENVOV1 and ENVOV2 polypeptides. (a) The coding potential of the ENVOV1 and ENVOV2 polypeptides was confirmed by overexpression followed by Western blot analysis using antibody against Rauscher MuLV gp69/71 env polypeptide. (b) The cellular distribution of the overexpressed ENVOV1 and ENVOV2 polypeptides was examined by immunocytochemistry using antibody against Rauscher MuLV gp69/71 polypeptide, and their membrane staining pattern was evident. The cells transfected with a blank plasmid serves as a negative control (mock). DAPI (4′,6-diamidino-2-phenylindole).
