Research Article

Tropism, Cytotoxicity, and Inflammatory Properties of Two Envelope Genes of Murine Leukemia Virus Type-Endogenous Retroviruses of C57BL/6J Mice

Figure 3

Production of pseudotype LacZ-MuLV virions. Presence of the pseudotype LacZ-MuLV virus particles in culture supernatants of the GP2-293 packaging cells was confirmed by detection of the env polypeptides using antibody against Rauscher MuLV gp69/71. Arrow indicates the env polypeptides. Supernatants collected from cells transfected with a blank plasmid serves as a negative control (mock).
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