Tropism, Cytotoxicity, and Inflammatory Properties of Two Envelope Genes of Murine Leukemia Virus Type-Endogenous Retroviruses of C57BL/6J Mice
Figure 4
Cytopathic effects of the ENVOV1 and ENVOV2 polypeptides. (a) and (b) Cytotoxic property of the ENVOV2 polypeptide, but not ENVOV1 polypeptide, was observed during cytotoxicity assay by measurement of lactate dehydrogenase release and morphologic examination of cells (200x magnification). The degree of cytotoxicity was normalized to the no DNA (Panel (a)). (c) ENVOV2 polypeptide’s inhibitory effect on cell proliferation was demonstrated by MTT assay, which measures cell viability, following overexpression. All experiments were performed in triplicate. * and ** indicate statistical significance (*; **).
