Tropism, Cytotoxicity, and Inflammatory Properties of Two Envelope Genes of Murine Leukemia Virus Type-Endogenous Retroviruses of C57BL/6J Mice

<table>Effects of ENV<sub>OV1</sub> and ENV<sub>OV2</sub> polypeptides on expression of inflammatory mediators. (a) and (b) The effects of the ENV<sub>OV1</sub> or ENV<sub>OV2</sub> polypeptide in RAW264.7 on the expression of various inflammatory mediators are presented. Differential modulation potentials for inflammatory mediators were observed between ENV<sub>OV1</sub> and ENV<sub>OV2</sub> polypeptides. The densitometric value of each inflammatory mediator was normalized to <svg height="16.6625" id="M22" style="vertical-align:-3.56265pt" version="1.1" viewbox="0 0 10 16.6625" width="10" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>-actin, and a graph was formulated. Three different forms of negative control were included in this experiment: no DNA, pcDNA4 (blank pcDNA4/HisMax plasmid), and GR11 (similar insert size as ENV<sub>OV1</sub> and ENV<sub>OV2</sub>: mouse glucocorticoid receptor in pcDNA4/HisMax). The assay was performed in triplicate. * and ** indicate statistical significance (*<svg height="11.375" id="M23" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 50.549999 11.375" width="50.549999" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>).</table>

Mediators of Inflammation

fig5

Figure 5

Figure 5: Tropism, Cytotoxicity, and Inflammatory Properties of Two Envelope Genes of Murine Leukemia Virus Type-Endogenous Retroviruses of C57BL/6J Mice 

Figure 5 | Tropism, Cytotoxicity, and Inflammatory Properties of Two Envelope Genes of Murine Leukemia Virus Type-Endogenous Retroviruses of C57BL/6J Mice 