Nuclear Factor-Kappa B Activity Regulates Brain Expression of P-Glycoprotein in the Kainic Acid-Induced Seizure Rats
Figure 3
Pretreatment of PDTC, but not DEX, decreases KA induced NF-κB activation. (a) Representative photos of immunohistochemistry staining with anti- NF-κB p65 antibody (×400). NF-B expression and distribution pattern was detected in the rat brains of each group 24 h after KA or vehicle micro-injection. The NF-B p65 immunoreactive cell number increased as well as more nuclear staining was observed in the EP group (EP) compared to the control rat (NS). By contrast with EP group, the number of NF-B p65 positive cells were decreased in the group pre-treated with PDTC (PDTC), but not in the group with DEX pretreatment (DEX). (b) Representative photos of immunohistochemistry staining with antiphospho-NF-B p65 antibody (×400). Activated NF-κB in the rat brains of each group was detected 24 h after KA or vehicle micro-injection. The number of Phospho-p65 positive cells significantly increased 24 h after KA injection (EP) compared with that in NS group. Compared to the EP group, activated NF-B signal was significantly decreased in hippocampus CA3 area and amygdaloid nuclear complex of the rats pre-treated with PDTC (PDTC), but not in the rats with DEX pretreatment (DEX). # versus NS group; ## versus NS group; * versus EP group; *# versus EP group CA3, hippocampus CA3 area; DG: dentate gyrus; AMYG: amygdaloid nuclear.
