LPS Counter Regulates RNA Expression of Extracellular Proteases and Their Inhibitors in Murine Macrophages

Figure 5

Schematic diagram of expected  𝑃-values based on fold change and GPR analyses. The left side of the diagram shows a hypothetic GPR analysis of two groups (1 & 2) with a general and equal increase in gene expression levels in group 2 compared to group 1. In this case it is the single gene “E” that does not change its expression level that receives the lowest  𝑃-values. This result is based on the GPR algorithm where all genes are normalized to each other. The right side of the diagram shows the same scenario but analyzed using a classical fold change statistical analysis (e.g., Mann-Whitney test). As depicted, these two methods, although both mathematically consistent, give rise to different results. * indicates a statistical significant change.