Human Mast Cells (HMC-1 5C6) Enhance Interleukin-6 Production by Quiescent and Lipopolysaccharide-Stimulated Human Coronary Artery Endothelial Cells
Figure 6
Histamine and LPS induce expression of BMP-2 mRNA. Endothelial cells were incubated with medium (control), histamine, histamine + cetirizine (a), or with 10 or 100 ng/mL LPS (b) for 2 h. Total RNA was extracted, reverse transcribed and, analyzed using specific primer sets for BMP-2 using real-time RT-PCR with β-actin as the housekeeping gene (a and b). Results were analyzed and normalized using β-actin and expressed as fold changes over the control. (c) Endothelial cells were incubated with medium, histamine, LPS, or histamine + LPS for 2 or 24 h, and Western blotting was carried out to determine the expression of BMP-2 protein as described in details in the Section 2.
