Disruption of the ECM33 Gene in Candida albicans Prevents Biofilm Formation, Engineered Human Oral Mucosa Tissue Damage and Gingival Cell Necrosis/Apoptosis
Figure 7
C. albicans downregulated basement membrane protein production. Total proteins were extracted from the infected EHOMs and analyzed by western blotting for the presence of laminin 5 and type IV collagen. (a) Representative gel of three separate experiments. Unstimulated tissue was used as a control (Ctrl). Protein production modulation was determined by band scanning using public domain NIH Image software. (b) Histograms are the means + SD . Statistical differences were found by comparing the values obtained from the infected and noninfected tissues. .
