Low Dose Theophylline Showed an Inhibitory Effect on the Production of IL-6 and IL-8 in Primary Lung Fibroblast from Patients with COPD

Figure 2

Increased mRNA and protein levels of interleukin-8 (IL-8) in lung fibroblasts from COPD patients. (a) Real-time RT-PCR was adopted to evaluate the transcription levels of IL-8, and the relative expression was calculated based on the expression of GAPDH. **𝑃=0.002, when compared to control group. (b) The relationship of IL-8 mRNA and FEV₁. (c) The protein level of IL-8 of primary lung fibroblasts from subjects with COPD. ELISA was adopted to measure the concentration of IL-8 in the cell-free supernatants. ***𝑃<0.001, when compared to control group. (d) The relationship of IL-8 secretion.

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