The Anti-Inflammatory Cytokine, Interleukin-10, Inhibits Inflammatory Mediators in Human Epithelial Cells and Mouse Macrophages Exposed to Live and UV-Inactivated Chlamydia trachomatis
Figure 3
Expression profiles of IL-8 and IL-6 produced in C. trachomatis-(CT-) infected HeLa cells noncumulative supernatants. HeLa cells (105 cells/mL/well) were seeded in 24-well plates. Twenty-four hours later cells were infected with C. trachomatis EBs (104 IFU/well) and after day 1 (a and b), day 2 (c and d), and day 3 (e and f) infection, cell-free culture supernatants were collected daily and replaced with fresh media (noncumulative). Human IL-8 and IL-6 cytokines were measured using cytokine-specific ELISA. Asterisk indicates significant difference (), and values were calculated by use of Student’s t-test. Each bar represents the mean ± SD of samples run in triplicates. The data are representative of two separate experiments.
