The Anti-Inflammatory Cytokine, Interleukin-10, Inhibits Inflammatory Mediators in Human Epithelial Cells and Mouse Macrophages Exposed to Live and UV-Inactivated Chlamydia trachomatis
Figure 4
The production of IL-6 and TNF were induced in a time-dependent fashion in C. trachomatis-(CT-) infected mouse J774 macrophages. J774 cells (105 cells/mL/well) were seeded in 24-well plates. Twenty-four hours later, cells were infected with C. trachomatis EBs (104 IFU/well) and cell-free cultured supernatants were collected at different time-points. The levels of IL-6 (a) and TNF (b) were measured in supernatants by cytokine-specific ELISA. For positive control, J774 cells (106 cell/mL) were stimulated with LPS (1 g/mL) (c) for various time-points. Asterisk indicates significant difference (), and values were calculated by use of Student’s t-test. Each bar represents the mean ± SD of samples run in duplicates. The data are representative of two separate experiments.
