The Anti-Inflammatory Cytokine, Interleukin-10, Inhibits Inflammatory Mediators in Human Epithelial Cells and Mouse Macrophages Exposed to Live and UV-Inactivated Chlamydia trachomatis
Figure 5
Recombinant IL-10 downregulates the production of IL-6, IL-8, and TNF in C. trachomatis-(CT-) infected J774 and HeLa cells. HeLa or J774 cells (105 cells/mL/well) were seeded in 24-well plates and 24 hr later, cells were infected with C. trachomatis EBs (104 IFU/well). After 2 days infection, various concentrations of recombinant IL-10 were added to cell cultures and supernatants were collected 1 day later to quantify the levels of human IL-6 and IL-8 (a) and mouse IL-6 and TNF (b) by ELISA. For positive control, HeLa and J774 cells were stimulated for 24 hr with LPS in the presence of various concentrations of IL-10, and human IL-8 and IL-6 (b) and mouse IL-6 and TNF (d) were determined from collected supernatants by ELISA. Asterisk indicates significant difference () and values were calculated by use of Student’s t-test. Each bar represents the mean ± SD of samples run in duplicates. The data are representative of two separate experiments.
