The Anti-Inflammatory Cytokine, Interleukin-10, Inhibits Inflammatory Mediators in Human Epithelial Cells and Mouse Macrophages Exposed to Live and UV-Inactivated Chlamydia trachomatis
Figure 6
UV-inactivated C. trachomatis induction of TNF and IL-6 levels in mouse J774 macrophages are downregulated by exogenous IL-10. J774 cells (105 cells/well) were stimulated for 24 hr with various concentrations of UV-inactivated C. trachomatis. The production of TNF and IL-6 was quantified in cell-free culture supernatants using cytokine-especific ELISAs. Shown are the dose-dependent TNF and IL-6 responses (a) and the downregulatory effect of IL-10 on these cytokines (b). Asterisk indicates significant difference (), and values were calculated by use of Student’s t-test. Each bar represents the mean ± SD of samples run in triplicates. The data are representative of two separate experiments.
