High-Mobility Group Box 1 Induces Calcineurin-Mediated Cell Hypertrophy in Neonatal Rat Ventricular Myocytes
Figure 1
Effect of different concentrations of HMGB1 (50 to 200 ng/mL) on NRVM protein synthesis and ANP expression over 24 hours. (a) NRVM protein synthesis was determined by measuring protein content per cell. (b) Expression of ANP protein in NRVMs was determined by Western blot analysis, and the integrated optical density (IOD) of ANP expression bands was analyzed. (c) Expression of ANP mRNA in NRVMs was determined by real-time RT-PCR. The bar graph represents three independent experiments. * versus control. Ctrl: control group.
