High-Mobility Group Box 1 Induces Calcineurin-Mediated Cell Hypertrophy in Neonatal Rat Ventricular Myocytes
Figure 3
Effect of CsA on NRVMs pretreated with HMGB1. CsA (1 μM) was applied for 1 h prior to a 200 ng/mL HMGB1 challenge in NRVMs. (a) NRVM protein synthesis was determined by protein content per cell. (b) Expression of ANP protein in NRVMs was determined by Western blot analysis; the integrated optical density (IOD) of ANP expression bands was analyzed. (c) Expression of ANP mRNA in NRVMs was determined by real-time RT-PCR. (d) Calcineurin activity was determined by a calcineurin assay kit. The bar graph represents three independent experiments. * versus control. # versus 200 ng/mL HMGB1 treated group. Ctrl: control group; CsA: cyclosporin A.
