Role of Macrophage Migration Inhibitory Factor in the Proliferation of Smooth Muscle Cell in Pulmonary Hypertension
Figure 6
MIF induced ERK1/2 and JNK phosphorylation in rat PASMCs. PASMCs were treated with MIF (100 ng/mL) for different time points. Significant ERK1/2 (A) and JNK (B) phosphorylation was induced after 15 min of MIF treatment. (A) Cell lysates were subjected to immunoblotting analysis using antibodies against phospho-ERK1/2 (p ERK1/2) and total ERK1/2 (a). Bar graph showed ERK1/2 protein levels obtained from quantitative densitometry analysis (b). (B) Cell lysates were subjected to immunoblotting analysis using antibodies against phospho-JNK (p JNK) and total JNK (a). Bar graph showed JNK protein levels obtained from quantitative densitometry analysis (b). . versus control.
