Role of Macrophage Migration Inhibitory Factor in the Proliferation of Smooth Muscle Cell in Pulmonary Hypertension
Figure 7
Effects of MIF inhibition on phosphorylation of ERK1/2 and JNK in rat PASMCs. MIF-stimulated ERK1/2 and JNK phosphorylation for 60 min was inhibited by MIF antagonist ISO-1 (50 μM). (A) Cell lysates were analyzed by immunoblotting using antibodies specific for phospho-ERK1/2 (p ERK1/2) and total ERK1/2 (a). Bar graph showed ERK1/2 protein levels obtained from quantitative densitometry analysis (b). (B) Cell lysates were analyzed by immunoblotting using antibodies specific for phospho-JNK (p JNK) and total JNK (a). Bar graph showed JNK protein levels obtained from quantitative densitometry analysis (b). . versus vehicle-treated control; versus groups treated with MIF alone.
