Flavonoid Naringenin: A Potential Immunomodulator for Chlamydia trachomatis Inflammation
Figure 4
Naringenin reduces the transcriptional activation of TLR2 and CD86 by C. trachomatis in mouse J774 macrophages. Macrophages (3 106 cells/mL) were left uninfected or infected with live C. trachomatis (3 105 IFU/well). After 2-day infection of macrophages with C. trachomatis (CT), naringenin (1 μg/mL) was added to macrophage cultures and 2 days later total RNA was extracted as described in Section 2. One step qRT-PCR was used to quantify the mRNA gene transcripts of TLR2 and CD86. is considered significant when compared to untreated cells (J774) and to cells treated with CT or CT + naringenin. Data shown is an average of triplicates run representative of two separate experiments.
