Flavonoid Naringenin: A Potential Immunomodulator for Chlamydia trachomatis Inflammation
Figure 6
Naringenin downregulates the phosphorylation of p38 MAPK in C. trachomatis infected mouse J774 macrophages. Macrophages (6 cells/well) were seeded in 6-well plates and then infected with C. trachomatis (3 105 IFU/well) or LPS (3 μg/well). After infection of macrophages, protein lysates were collected as indicated in Section 2. The presence of total p38 (p38) (internal control) and phosphorylated p38 (Pp38) was determined by western blotting. Shown are the band intensities for internal control and pp38 at different time points for macrophages treated with C. trachomatis (CT) and LPS (a). Blots shown in (b) and (c) were developed 1 h after exposing macrophages to CT or LPS in the presence and absence of naringenin. The 43 kDa Pp38 and p38 proteins were determined from a known biotinylated protein ladder.
