Activation of Protease-Activated Receptor 2-Mediated Signaling by Mast Cell Tryptase Modulates Cytokine Production in Primary Cultured Astrocytes
Figure 1
Effects of tryptase (Tt) and specific inhibitor of PAR-2 or MAPKs on the viability of astrocytes. For the dose-dependent studies astrocytes were treated with either culture medium only or various concentrations of tryptase (0.001, 0.01, 0.1, 1, and 10 μg/mL), FSLLRY-NH2 (FS, 200 or 400 μM), SB203580 (SB, 20 μM), PD98059 (PD, 20 μM), SP600125 (SP, 20 μM) and LY294002 (LY, 20 μM) for 24 h. Data are presented as the mean ± SEM in triplicate on four separate occasions. versus control groups (Con).
