Activation of Protease-Activated Receptor 2-Mediated Signaling by Mast Cell Tryptase Modulates Cytokine Production in Primary Cultured Astrocytes

Figure 6

Time courses of tryptase (Tt) on the expression of transforming growth factor-β (TGF-β) and ciliary neurotrophic factor (CNTF) in astrocytes. (a) Tryptase at 1 μg/mL was incubated with astrocytes at 37°C for 0, 30, 60, 120, and 240 min, respectively. Protein levels were detected by immunoblot analysis with specific antibodies. Western blots at the top of each panel are from a typical experiment. (b) Bar graphs are the quantified results expressed as mean ± SEM of TGF-β and CNTF levels from three independent experiments. ^∗∗𝑃<0.01, ^∗𝑃<0.05 versus 0 min.

(a)

(b)