Characterization of HIV-1 Infection and Innate Sensing in Different Types of Primary Human Monocyte-Derived Macrophages
Figure 4
Innate sensing of DNA in human MDMs via intracellular DNA sensors. MDMs were produced during a 7-day period through stimulation by four differentiation protocols. The MDMs were transfected with poly(dA : dT) (1.7 μg/mL) using lipofectamine2000, treated with lipofectamine alone, or left untreated (UT) for 6 hours before harvest of supernatants. Levels of CCL5 and CXCL10 were measured by ELISA. Data shown are means ± SEM from 6 donors from 2 separate experiments.
