Scutellarin Attenuates Hypertension-Induced Expression of Brain Toll-Like Receptor 4/Nuclear Factor Kappa B

Figure 3

Scutellarin suppresses hypertension-induced expression of brain Toll-like receptor 4 (TLR4). (a) Chronic hypertension significantly enhanced the expression of TLR4 in the cerebral cortex. (b) Double immunofluorescence labeling indicated that the majority of TLR4 (red) was colabeled with OX-42-positive (green) cells and fewer with glial fibrillary acid protein- or Neuronal Nuclei-positive cells. (c) Western immunoblot analysis of brains of hypertensive rats showed that scutellarin dose-dependently suppressed the expression of TLR4. ^∗𝑃<0.001 versus sham group; ^◆𝑃<0.001 versus NS group; ^&𝑃<0.001 versus low-dose group. (c) Scale bar = 200 μm (a) and 50 μm (b).