High Sugar Intake and Development of Skeletal Muscle Insulin Resistance and Inflammation in Mice: A Protective Role for PPAR-δ Agonism
Figure 6
Effects of dietary manipulation and GW0742 treatment on NF-B p65 translocation (a), ICAM-1 (b) and iNOS (c) protein expression in the mouse gastrocnemius. Protein expression was measured by Western blot analysis in gastrocnemius homogenates of mice fed with a standard (control) or HFCS diet (HFCS) in the absence or presence of GW0742 treatment (1 mg/kg/day) (; ). Densitometric analysis of the bands is expressed as relative optical density (O.D.), corrected for the corresponding tubulin contents, and normalized using the related control band. NF-B p65 subunit levels in cytosolic and nuclear fractions are expressed as nucleus/cytosol ratio normalized using the related control band. Data are of three separate experiments. * versus HFCS.
