The Role of Glucocorticoid Receptors in Dexamethasone-Induced Apoptosis of Neuroprogenitor Cells in the Hippocampus of Rat Pups
Figure 1
TUNEL staining of dentate gyrus (DG) P1 D1D2 pups treated with dexamethasone (Dex) or normal saline (NS) shows apoptotic cells stained brown (arrows). The Dex-treated D1D2 DG had more apoptotic cells (b) than NS control (a). High magnification photomicrographs of TUNEL-positive cells are shown in the upper left corner. TUNEL-positive cell counts revealed that the number of apoptotic cells in the Dex-treated D1D2 DG was increased when compared to that of NS control pups (, *) (c). Cleaved-caspase 3 staining of the DG from P1 D1D2 pups with or without Dex treatment showed apoptotic cells (arrows) ((d) and (e)). High magnification photomicrographs of cleaved-caspase 3-positive cells are shown in the upper left corner. The numbers of apoptotic cells in Dex-treated pups were increased compared to those of NS control (f) (, *). Magnification, 200x; scale bars, 100 μm; inset magnification, 400x. P1 D1D2: P1 pups receiving treatment on postnatal day 1 and sacrificed on day 2.
