SOCS3 Expression Correlates with Severity of Inflammation, Expression of Proinflammatory Cytokines, and Activation of STAT3 and p38 MAPK in LPS-Induced Inflammation In Vivo
Figure 1
Representative images and stereometric analysis of each experimental period (7, 15, and 30 days) for the LPS-injected tissues (experimental group). A single representative image of the control group is shown, as no important changes were noted in this group in the different experimental periods (data not shown). The general characteristics of the inflammatory reaction include increase in inflammatory-like cell density and vascular structures, and a decrease in the number of fibroblast-like cells and extracellular matrix content was observed in all experimental periods. The image of a control site depicts the placement of the 3240 μm2 grids on the submarginal area limited coronally by the apical border of the junctional epithelium and laterally by the tooth structure. Stereometric analysis indicates that LPS injections caused a sustained inflammatory reaction. The relative presence of inflammatory cells, vascular structures, fibroblasts, and extracellular matrix in 32400 μm2 area (schematically shown on (a)) was analyzed. The severity of inflammation was significantly higher in comparison to the control group throughout the 30-day experimental period. *Significant differences compared with control groups (, ANOVA, and post hoc Tukey for pairwise comparisons).
