Local Overexpression of Interleukin-11 in the Central Nervous System Limits Demyelination and Enhances Remyelination
Figure 4
Scheme and the effect of therapeutic overexpression of IL-11 on remyelination. (a) Mice were fed with cuprizone diet for 4 weeks before PBS/eGFP-LV/IL-11-LV was administered stereotactically in the brain. Cuprizone diet was continued for more 1 week, before returning mice to standard chow for another 2 weeks. Panel (b) shows representative images of coronal brain sections from all groups, depicting myelinated area (LFB) and density of mature OLs (CC1). Quantitative analysis reveals that therapeutic overexpression of IL-11 (i) enhances remyelination, as illustrated by luxol fast blue staining depicting a larger myelinated area in the IL-11-LV treated group (c), and (ii) facilitates the maturation of OL’s as revealed by a higher density of CC1+ mature oligodendrocytes in the IL-11-LV treated group (d). Data is presented as mean ± SEM ( animals for the cuprizone treated groups; for the healthy control group). Statistical significance was analyzed by one-way ANOVA followed by Bonferroni’s multiple comparison test. , as compared to the healthy control group; *, **, as compared to the 5 wk CUP 2 wk RM + PBS. Quantification was performed in the corpus callosum of at least three coronal brain sections per mouse, chosen between anterioposterior coordinates from 0.3 to −1.5 mm in reference to the bregma. Scale bar: 200 µM.
