Flavonoids and 5-Aminosalicylic Acid Inhibit the Formation of Neutrophil Extracellular Traps
Figure 5
NET formation after PMA stimulation visualized by fluorescence (a) and scanning electron microscopy (b). Upper row shows unstimulated neutrophils; the lower row shows PMA stimulated neutrophils releasing NETs. The scale bar represents 10 μm (fluorescence microscopy) and 5 μm (scanning electron microscopy). (a) Immunohistochemical staining of neutrophils. Green: DNA staining with SYTOXgreen; red: MPO; overlay: SYTOXgreen and MPO. NET release of freshly isolated neutrophils was induced by 10 nM PMA and the samples were fixed after 3 h. Arrows indicate NET fibres and NETotic cells, defined as NETs. (b) Scanning electron microscopy of NET formation induced by 20 nM PMA and fixed after 3.5 h.
