Downregulation of NOX4 Expression by Roflumilast N-Oxide Reduces Markers of Fibrosis in Lung Fibroblasts

Figure 1

Effect of BLM and RNO on HLF proliferation and collagen synthesis. HLF were preincubated or not with RNO (2 nM) for 30 min and then treated with BLM (50 mU/mL). (a) Cell proliferation was evaluated on HLF from three different donors after 24 h by measuring [³H]-thymidine incorporation. Results are expressed as [³H]-thymidine incorporation (dpm) per well. Each sample was run in quadruplicate. Data are mean ± SD of 3 experiments. ^∗𝑃<0.05 versus control (C); ^#𝑃<0.05 versus BLM. (b) For determination of [³H]-proline incorporation into collagen and noncollagen proteins, media were harvested after 48 h of treatment. Results are presented as percentage of untreated control. Data are mean ± SD of 3 experiments each in quadruplicate. ^∗𝑃<0.05 versus control (C); ^#𝑃<0.05 versus BLM.

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