Downregulation of NOX4 Expression by Roflumilast N-Oxide Reduces Markers of Fibrosis in Lung Fibroblasts
Figure 2
BLM-induced oxidative stress in HLF. (a) HLF were treated with various concentrations of BLM, and the generation of ROS was evaluated at 4 (white bar) and 24 (black bar) h. ROS levels were measured using the probe 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA). ROS production was determined over a 60 min period. (b) The effect of RNO on the formation of ROS was evaluated in HLF from 3 different cell lines preincubated for 30 min in presence or absence of RNO (2 nM) and then treated with BLM (50 mU/mL) for 24 h. The results are expressed as arbitrary units of fluorescence measured on a PerkinElmer Applied Biosystems fluorescence plate reader (excitation 490 nm; emission 535 nm). Data are mean SD of 3 experiments. versus control (C); versus control (C); versus BLM.
