Downregulation of NOX4 Expression by Roflumilast N-Oxide Reduces Markers of Fibrosis in Lung Fibroblasts

Figure 5

Effect of BLM and RNO on NOX4 expression in HLF. (a) Cells were preincubated or not with RNO (2 nM) and then treated for 24 h with BLM (50 mU/mL). Protein expression of NOX4 was analyzed by Western blotting. (b) Quantification of proteins was expressed as a ratio to β-actin. Data are mean ± SD of 3 experiments. ^∗∗𝑃<0.01 versus control (C); ^##𝑃<0.01 versus BLM. (c) NOX4 gene expression in HLF was evaluated by real-time PCR. The isolated mRNA samples were analyzed using the specific primers and compared with β-actin (housekeeping gene) levels. Data are mean ± SD of 3 experiments. ^∗𝑃<0.05 versus control (C); ^#𝑃<0.05 versus BLM.