Downregulation of NOX4 Expression by Roflumilast N-Oxide Reduces Markers of Fibrosis in Lung Fibroblasts
Figure 6
Effect of 8-epi-PGF2α (ISO) and RNO on ROS production by HLF. (a) HLF were treated for 24 h with various concentrations of 8-epi-PGF2α. ROS levels were measured as described in Section 2.4. ROS production was determined over a 60 min period. (b) Cells were preincubated for 30 min in presence or absence of RNO (2 nM) and then treated with 8-epi-PGF2α (10−8 M) for 24 h. The results are expressed as arbitrary units of fluorescence. Data are mean SD of 3 experiments. versus control (C); versus 8-epi-PGF2α.
