Downregulation of NOX4 Expression by Roflumilast N-Oxide Reduces Markers of Fibrosis in Lung Fibroblasts

Figure 8

Effect of 8-epi-PGF_2α (ISO) and RNO on HLF proliferation and collagen synthesis. (a) Cells were preincubated for 30 min in presence or absence of RNO (2 nM) and then stimulated with 8-epi-PGF_2α (10⁻⁸M). Cell proliferation was evaluated after 24 h by measuring [³H]-thymidine incorporation in HLF. Results are expressed as [³H]-thymidine incorporation (dpm) per well. Each sample was run in quadruplicate. Data are mean ± SD of 3 experiments. ^∗∗𝑃<0.01 versus control (C); ^##𝑃<0.01 versus 8-epi-PGF_2α (ISO). (b) Media were harvested after 48 h of treatment for determination of [³H]-proline incorporation into collagen and noncollagen proteins. Results (collagen/total protein) are presented as percentage of untreated control. Data are mean ± SD of 3 experiments. ^∗𝑃<0.05 versus control (C); ^#𝑃<0.05 versus 8-epi-PGF_2α (ISO).

(a)

(b)