Novel Biphasic Role of Resolvin D1 on Expression of Cyclooxygenase-2 in Lipopolysaccharide-Stimulated Lung Fibroblasts Is Partly through PI3K/AKT and ERK2 Pathways
Figure 4
COX-2 protein expression is partly through the activation of ERK1/2 and PI3K/AKT signaling pathways in primary rat lung fibroblasts stimulated with LPS. ((a1), (a2)) COX-2 protein expression induced by LPS in primary rat lung fibroblasts at 6 hours (the first COX-2 expression peak) was suppressed by inhibitors of PI3K/AKT (LY294002) and ERK1/2 (UO126) (ā versus control, LPS + LY294002, LPS + UO126, LY294002, and UO126 groups). There is no significant change between control, LY294002, and UO126 groups (). ((b1), (b2)) COX-2 protein expression induced by LPS in primary rat lung fibroblasts at 48 hours (the second COX-2 expression peak) was not suppressed by inhibitors of PI3K/AKT (LY294002) and ERK1/2 (UO126) (ā versus control, LY294002, and UO126 groups). There is no significant change between LPS, LPS + LY294002 and LPS + UO126 groups (). All experiments were repeated in triplicate.
