The Effect of IL-4 Gene Polymorphisms on Cytokine Production in Patients with Chronic Periodontitis and in Healthy Controls
Table 3
Sample medians for cytokine levels expressed by mitogens (PWM, P + C), by HSP70, and by selected bacteria in comparison to patients with periodontitis () and healthy controls () in division to groups by IL-4 genotypes (polymorphisms).
(a)
Locus
Periodontitis patients
Healthy controls
IL-4-590
IL-4VNTR
IL-4-590
IL-4VNTR
Polymorphism
CC
CT + TT
11
12 + 22
CC
CT + TT
11
12 + 22
(Number of patients)
(30)
(16 + 1)
(28)
(17 + 1)
(8)
(5 + 2)
(10)
(5 + 0)
Cytokines [pg/mL]
Median
(Min/max)
Median
(Min/max)
Median
(Min/max)
Median
(Min/max)
Median
(Min/max)
median
(Min/max)
Median
(Min/max)
Median
(Min/max)
IFNg_PWM
116.7
(0/7816.5)
180.6
(0.8/3898.5)
116.8
(0.2/7816.5)
147.6
(0/3898.5)
4.5
(0.5/983.9)
178.6
(2.2/1073.7)
4.5
(0.5/983.9)
178.6
(2.2/1073.7)
IFNg_P + C
92.8
(0.3/7215)
71.2
(0.3/1280.4)
113.6
(0.6/7215)
56.0
(0.3/1280.4)
126.9
(1.1/1009.9)
81.6
(2/1081)
126.9
(1.1/1009.9)
81.6
(2/1081)
IFNg_HSP70
1.5
(0/7282)
0.3
(0/781.9)
1.1
(0/7282)
0.3
(0/781.9)
1.1
(0.1/550.5)
0.3
(0.2/552.4)
1.1
(0.1/550.5)
0.3
(0.2/552.4)
IFNg_Pg
0.9
(0/633.3)
1.6
(0/9.2)
0.7
(0/633.3)
2.1
(0/9.2)
0.6
(0/4.8)
0.4
(0.2/1)
0.5
(0/4.8)
0.4
(0.2/1)
IFNg_Aa
2.9
(0/230.1)
1.1
(0/405.2)
2.9
(0/230.1)
1.1
(0/405.2)
1.0
(0/119.1)
2.1
(0.2/240)
1.0
(0/119.1)
3.2
(0.2/240)
IFNg_Tf
2.6
#
(0.1/633.3)
#
0.4
(0/9.9)
2.1*
(0.1/633.3)*
0.4
(0/9.9)
1.6
(0/89.4)
0.3
(0.1/99.5)
0.4
(0/89.4)
0.4
(0.1/99.5)
IFNg_PI
0.8
(0/238.7)
0.2
(0/1.9)
0.8
(0/238.7)
0.3
(0/2.2)
0.7
(0/61.1)
0.2
(0.1/809.9)
0.3
(0/61.1)
0.2
(0.1/809.9)
IFNg_unstim.
0.7
(0/21.9)
0.1
(0/8.6)
0.6
(0/21.9)
0.1
(0/8.6)
0.0
(0/91)
0.1
(0/7)
0.0
(0/91)
0.1
(0.1/0.4)
IL-10_PWM
63.5
(0/3447)
60.3
(0/518.6)
90.3
(0/3447)
55.1
(0/518.6)
76.0
(0/1017)
85.7
(37.4/326)
76.0
(0/1017)
180.7
(37.4/326)
IL-10_P + C
37.2
(0/866.7)
30.4
(0/1732.9)
41.0
(0/866.7)
27.6
(0/1732.9)
125.9
(8.8/497.2)
105.1
(26.3/537.7)
125.9
(8.8/497.2)
105.1
(26.3/537.7)
IL-10_HSP70
5.1
(0/581.3)
1.7
(0/271.7)
5.6
(0/581.3)
3.2
(0/271.7)
9.7
(1.3/250.3)
10.3
(3.9/215.4)
10.4
(1.3/250.3)
4.8
(3.9/215.4)
IL-10_Pg
2.5
(0/164.5)
2.3
(0/81.9)
1.8
(0/164.5)
3.6
(0/81.9)
0.5
(0/45.7)
0.3
(0.1/9)
0.5
(0/45.7)
0.3
(0.1/6.2)
IL-10_Aa
28.8
(0.8/823.6)
25.2
(0.1/589.9)
36.8
(0.8/823.6)
24.0
(0.1/589.9)
26.6
(11.2/303.1)
58.2
(4.4/663.1)
26.6
(4.4/303.1)
92.5
(10.7/663.1)
IL-10_Tf
18.2
(0.1/374.8)
20.6
(0.1/374.8)
134.2*
(4.3/320.1)*
52.9
(0.6/151.6)
92.6
(0.6/320.1)
63.8
(0.7/151.6)
IL-10_PI
25.0
#
(0/207.2)
#
2.7
(0/27.6)
22.7*
(0/207.2)*
3.0
(0/81.9)
9.2
(0/173.6)
3.7
(0.4/29.1)
5.7
(0/173.6)
5.5
(1.1/29.1)
IL-10_unstim.
2.7
(0/14.7)
0.4
(0/7.2)
2.0
(0/14.7)
0.6
(0/7.2)
0.4
(0/11)
2.1
(0.7/6.2)
0.7
(0/11)
2.8
(0.7/6.2)
IL-17_PWM
8.0
(0/2200)
15.0
(5.1/1248.5)
8.5
(0.5/2200)
13.5
(0/1248.5)
47.2
(0.8/2064.1)
127.0
(2.3/947.3)
47.2
(0.8/2064.1)
127.0
(3/947.3)
IL-17_P + C
34.4
(1.1/2200)
62.1
(2.8/2200)
50.2
(1.1/2200)
42.2
(1.5/2200)
239.2
(2.8/2200)
140.4
(0.7/2376.8)
239.2
(0.7/2200)
140.4
(1.1/2376.8)
IL-17_HSP70
6.3
(0/1107.5)
1.2
(0/968.6)
4.0
(0/1107.5)
1.4
(0/968.6)
7.2
(0.1/349.5)
0.3
(0.1/737.9)
7.2
(0.1/349.5)
0.3
(0.1/737.9)
IL-17_Pg
1.1
(0/543.1)
0.4
(0/12.9)
1.0
(0/543.1)
1.2
(0/12.9)
0.1
(0/6.9)
0.3
(0.1/0.6)
0.1
(0/6.9)
0.3
(0.1/0.4)
IL-17_Aa
2.6
(0.8/88.3)
1.1
(0.1/45.1)
2.4
(0.8/88.3)
1.9
(0.1/45.1)
0.3
(0/10.2)
1.2
(0/72.2)
0.4
(0/10.2)
1.2
(0/72.2)
IL-17_Tf
1.6
(0/2064.1)
1.5
(0/22.6)
1.5
(0/2064.1)
2.1
(0/22.6)
0.7
(0/11.6)
0.4
(0/168.9)
0.3
(0/11.6)
1.4
(0/168.9)
IL-17_PI
1.5
(0/62.1)
0.0
(0/6.3)
1.5
(0/62.1)
0.1
(0/6.3)
0.4
(0/10.2)
0.4
(0/2.2)
0.3
(0/10.2)
1.5
(0/2.2)
IL-17_unstim.
1.2
(0/5.1)
0.5
(0/6.3)
1.0
(0/5.1)
0.8
(0/6.3)
0.2
(0/2.8)
0.3
(0/22.7)
0.2
(0/22.7)
0.3
(0/2.5)
IL-1 alfa_PWM
141.3
(0/2400)
160.7
(22/7780)
152.0
(0/2400)
158.7
(22/7780)
231.3
(0/967)
258.7
(112/774.1)
197.1
(0/967)
412.2
(212.2/774.1)
IL-1 alfa_P + C
140.0
(2.3/4701.5)
124.6
(3.6/1643.5)
140.6
(4.3/4701.5)
124.2
(2.3/1643.5)
159.9
(15.4/1153.3)
247.1
(121.9/436.8)
159.9
(15.4/1153.3)
261.1
(242.5/436.8)
IL-1 alfa_HSP70
32.8
(0/1139.8)
13.0
(0/187.4)
52.3
(0/1139.8)
13.0
(0/187.4)
24.1
(6.4/398.9)
124.0*
(43.3/496.4)*
31.1
(6.4/398.9)
124.0
(43.3/496.4)
IL-1 alfa_Pg
47.5
(4.3/635.4)
44.0
(1.9/339)
47.5
(4.4/635.4)
44.7
(1.9/339)
38.4
(6.8/372.5)
69.9
(53.8/263.5)
44.2
(6.8/372.5)
63.8
(53.8/263.5)
IL-1 alfa_Aa
97.2
(11.2/724.2)
99.3
(2.8/1745.6)
97.2
(13.3/724.2)
110.5
(2.8/1745.6)
129.7
(13/238.7)
350.3*
(108.3/821.5)*
133.5
(13/238.7)
405.3*
(167.8/821.5)*
IL-1 alfa_Tf
106.3
(0/450.7)
37.7
(3.3/188.4)
106.3
(0/450.7)
46.7
(3.3/194.9)
121.4
(18.6/720.9)
236.9
(0.2/465.6)
111.5
(18.6/720.9)
355.3
(0.2/465.6)
IL-1 alfa_PI
134.2
(15.9/248.2)
17.0
(1.9/238.7)
133.3
(15.9/248.2)
34.4
(1.9/238.7)
84.4
(11.9/218.5)
242.3
(11.9/346.4)
84.6
(11.9/346.4)
242.3
(11.9/287)
IL-1 alfa_unstim.
17.4
(0/185.5)
6.8
(0/397.3)
18.8*
(0/185.5)*
9.0
(0/397.3)
20.8
(2.3/120.6)
140.2*
(5.9/308.8)*
22.5
(2.3/178.1)
140.2
(5.9/308.8)
IL-1 beta_PWM
583.3
(0/2586)
267.1
(0/1597)
655.9*
(0/2586)*
214.2
(0/1597)
676.4
(2.6/1600)
839.7
(100.9/1700)
676.4
(2.6/1600)
839.7
(285.3/1700)
IL-1 beta_P + C
533.3
(1.3/2650)
278.3
(1.6/1600)
600.2*
(2.6/2650)*
266.5
(1.3/1600)
695.2
(47.2/1600)
610.5
(168.2/1657)
571.9
(47.2/1600)
733.8
(324.8/1657)
IL-1 beta_HSP70
141.5
(0/1600)
34.7
(0/616.1)
228.9*
(0/1600)*
34.7
(0/616.1)
93.6
(44.4/1600)
335.9
(183.2/1338.1)
143.4
(44.4/1600)
335.9
(198.5/1338.1)
IL-1 beta_Pg
33.2
(0/851.4)
13.8
(0/600.4)
33.2
(0/851.4)
16.3
(0/600.4)
22.9
(3.7/106)
8.2
(2.4/289.7)
22.9
(2.9/289.7)
8.2
(2.4/189.5)
IL-1 beta_Aa
533.3
(0/4216.5)
482.3
(0/1600)
533.3*
(0/4216.5)*
405.2
(0/1600)
439.5
(47.7/1271.5)
483.6
(167.5/1531.1)
439.5
(47.7/1271.5)
748.0
(344.3/1531.1)
IL-1 beta_Tf
516.8*
(0/2952)*
109.6
(0/952.7)
533.3
#
(0/2952)
#
101.8
(0/952.7)
521.6
(47.7/1600)
448.5
(0/1700)
479.4
(47.7/1600)
558.8
(0/1700)
IL-1 beta_PI
251.0
(0/552.8)
63.8
(0/578.6)
244.7
(0/552.8)
68.7
(0/578.6)
302.9
(145.5/985)
342.0
(44.4/748.2)
302.9
(145.5/985)
390.8
(44.4/748.2)
IL-1 beta_unstim.
83.5*
(0/968.5)*
21.9
(0/533.3)
91.8*
(0/968.5)*
16.6
(0/533.3)
66.0
(9.5/621.8)
195.2
(21.5/415.6)
66.0
(9.5/621.8)
247.7
(21.5/415.6)
(b)
Locus
Periodontitis patients
Healthy controls
IL-4-590
IL-4VNTR
IL-4-590
IL-4VNTR
Polymorphism
CC
CT + TT
11
12 + 22
CC
CT + TT
11
12 + 22
Number of patients
(30)
(16 + 1)
(28)
(17 + 1)
(8)
(5 + 2)
(10)
(5 + 0)
Cytokines [pg/mL]
Median
(Min/max)
Median
(Min/max)
Median
(Min/max)
Median
(Min/max)
Median
(Min/max)
Median
(Min/max)
Median
(Min/max)
Median
(Min/max)
IL-4_PWM
3.6
(0/163.6)
3.9
(0/17.8)
3.7
(0/163.6)
3.4
(0/17.8)
4.3
(0.6/163.6)
3.6
(0.7/9.9)
4.3
(0.6/163.6)
3.6
(0.7/9.9)
IL-4_P + C
3.7
(0/40.7)
4.4
(0/25.7)
4.0
(0/40.7)
4.0
(0/25.7)
0.9
(0/20.1)
2.2
(0.7/19.9)
0.9
(0/20.1)
2.2
(0.7/11.9)
IL-4_HSP70
0.7
(0/6.5)
0.6
(0/5.5)
0.0
(0/6.5)
1.2
(0/5.5)
1.2
(0/3.1)
1.6
(0.7/2.3)
1.2
(0/3.1)
1.6
(0.7/2.3)
IL-4_Pg
0.5
(0/12.9)
0.1
(0/7.7)
0.1
(0/12.9)
0.1
(0/7.7)
0.7
(0/96.6)
0.7
(0/1.2)
0.7
(0/96.6)
0.7
(0.6/1.2)
IL-4_Aa
0.4
(0/11.2)
0.1
(0/3.7)
0.1
(0/4.7)
0.1
(0/11.2)
1.9
(0.7/113.8)
0.7
(0/4.5)
1.9
(0.7/113.8)
0.7
(0/4.5)
IL-4_Tf
1.8
(0/113.8)
0.8
(0/3.7)
0.1
(0/113.8)
1.6
(0/96.6)
1.2
(0/5.7)
1.2
(0.7/2.6)
1.2
(0/5.7)
1.2
(0.7/2.6)
IL-4_PI
0.7
(0/4)
0.0
(0/3.7)
0.3
(0/4)
0.6
(0/3.7)
0.7
(0/4.1)
0.7
(0/1.9)
0.7
(0/4.1)
0.7
(0/1.9)
IL-4_unstim.
0.0
(0/3.7)
0.0
(0/3.3)
0.0
(0/3.7)
0.0
(0/3.3)
0.9
(0/2.8)
0.7
(0/4.5)
0.9
(0/4.5)
0.7
(0/2.6)
IL-5_PWM
5.7
(0/1882)
3.7
(0/98.9)
8.4
(0/1882)
1.4
(0/98.9)
7.3
(0.6/212.4)
10.6
(1.8/143)
7.3
(0.6/212.4)
10.6
(1.8/112.4)
IL-5_P + C
8.7
(0/230.1)
9.5
(0/375.5)
9.0
(0/230.1)
6.9
(0/375.5)
20.0
(2.3/226.2)
9.2
(1.2/403.2)
20.0
(2.3/403.2)
9.2
(1.2/169.3)
IL-5_HSP70
1.5
(0/64.8)
0.3
(0/61)
1.0
(0/64.8)
0.6
(0/61)
3.8
(0.6/158)
6.0
(1.8/16.9)
4.3
(0.6/158)
5.2
(1.8/16.9)
IL-5_Pg
1.0
(0/322.7)
1.2
(0/8.1)
0.9
(0/322.7)
1.2
(0/8.1)
3.0
(0.6/6)
3.8
(3.2/7.7)
3.5
(0.6/7.7)
3.7
(3.2/6)
IL-5_Aa
1.7
(0/12.5)
1.0
(0/7.9)
1.5
(0/12.5)
1.2
(0/7.9)
3.3
(2.2/5.2)
5.2
(0/6)
3.3
(2.2/6)
5.2
(0/6)
IL-5_Tf
1.9
(0/212.4)
0.6
(0/3.7)
1.8
(0/212.4)
1.2
(0/3.9)
2.0
(1.2/5.2)
2.9
(1.2/6)
2.4
(1.2/6)
2.9
(1.2/6)
IL-5_PI
1.5
(0/28.6)
0.6
(0/3.7)
1.7
(0/28.6)
0.6
(0/3.7)
2.4
(0.6/4.2)
3.4
(0.6/6.9)
2.9
(0.6/4.7)
3.4
(0.6/6.9)
IL-5_unstim.
0.7
(0/8.1)
0.7
(0/7.9)
0.6
(0/8.1)
0.9
(0/7.9)
2.5
(0/6)
3.4
(0/6.9)
2.5
(0/6)
3.4
(1.8/6.9)
IL-6_PWM
3500.0
(0/5960)
3500.0
(0/4699)
3500.0
(5.4/5960)
3500.0
(0/4699)
2143.1
(0/4824.3)
3281.8
(130.5/5200)
2143.1
(0/5200)
3281.8
(1186.1/4272.5)
IL-6_P + C
3196.5
(0/6457)
2280.7
(44.7/5528.5)
3500.0
(9.3/6457)
2267.5
(0/5528.5)
2876.5
(211.1/4900)
1593.1
(123.2/5193.4)
2876.5
(211.1/5193.4)
1593.1
(123.2/4931.7)
IL-6_HSP70
773.9
(3.3/4207)
141.4
(2.7/5833.5)
1143.1
(3.3/4207)
51.9
(2.7/5833.5)
888.5
(165.6/3500)
2040.7
(167.5/4673.4)
888.5
(165.6/4673.4)
2040.7
(167.5/3111.3)
IL-6_Pg
19.8
(0/4191.6)
10.6
(0.4/3577.2)
19.8
(0/4191.6)
11.5
(0.4/3577.2)
13.8
(0/791)
6.5
(0.3/2438.4)
13.8
(0/2438.4)
6.5
(0.3/1675.2)
IL-6_Aa
3500.0
(27.5/6667)
3500.0
(71.9/7362.5)
3500.0
(839/6667)
3500.0
(27.5/7362.5)
3500.0
(108/4686)
2076.7
(258.5/3600)
3366.9
(108/4686)
2076.7
(1802.6/3600)
IL-6_Tf
3500.0
(25.1/6760)
1035.3
(1.4/4916)
3500.0*
(108/6760)*
913.9
(1.4/4916)
3457.2
(33.9/4203.3)
2037.2
(0.2/3500)
3449.9
(33.9/4203.3)
2037.2
(0.2/3500)
IL-6_PI
1052.6*
(336.1/4032)*
350.9
(0.7/3521.6)
1102.1*
(336.1/4032)*
585.5
(0.7/3521.6)
3011.5
(6.2/4686)
324.3
(87.5/1937.7)
2387.8
(6.2/4686)
324.3
(87.5/1337)
IL-6_unstim.
214.8
(2/3200.5)
20.0
(1/2051.9)
227.2*
(2/3200.5)*
8.0
(1/2051.9)
468.4
(33.9/1796.2)
558.5
(14.2/2046.3)
418.9
(14.2/1796.2)
601.9
(25.2/2046.3)
TNF alfa_PWM
1085.9
(2.8/3701)
1002.3
(0/3880)
1250.1
(3.5/3701)
963.4
(0/3880)
1506.6
(3.5/2800)
1209.1
(85.1/2800)
1494.2
(3.5/2800)
1209.1
(85.1/2800)
TNF alfa_P + C
1071.5
(0/3871)
450.2
(74/2800)
1127.2
(7.3/3871)
442.1
(0/2800)
1579.4
(35.6/2800)
1531.3
(22.9/2800)
1596.6
(35.6/2800)
1531.3
(22.9/2800)
TNF alfa_HSP70
32.1
(2.6/3494.6)
11.6
(2.3/2800)
92.6
(2.6/3494.6)
10.6
(2.3/2800)
63.4
(9.6/1249.1)
152.2
(11.8/1656.7)
87.6
(9.6/1656.7)
152.2
(11.8/432)
TNF alfa_Pg
20.6
(0.5/612.2)
13.6
(1.9/576.6)
21.1
(0.5/612.2)
13.3
(1.9/576.6)
2.1
(0.3/54.6)
0.8
(0.2/146.3)
2.1
(0.2/146.3)
0.8
(0.4/51.8)
TNF alfa_Aa
407.1
(10.7/4760)
462.2
(3.7/3880)
510.8
(10.7/4760)
441.8
(3.7/3880)
933.0
(104.4/3569.7)
527.6
(51.9/4347.3)
933.0
(51.9/3569.7)
527.6
(235.7/4347.3)
TNF alfa_Tf
650.9
(4.2/2845.4)
179.7
(2.1/2393.9)
687.5*
(104.4/2845.4)*
147.2
(2.1/2393.9)
899.8
(8.5/1813.5)
387.6
(0.1/965.3)
886.2
(8.5/1813.5)
387.6
(0.1/965.3)
TNF alfa_PI
74.0
(8.3/1448.7)
39.1
(1.2/182.6)
73.4
(8.3/1448.7)
55.0
(1.2/297.7)
80.5
(22.2/130.7)
38.2
(9.5/223)
73.4
(10.4/130.7)
38.2
(9.5/223)
TNF alfa_unstim.
14.0
(1.8/2336.7)
6.6
(1.7/91.8)
16.7
(1.8/2336.7)
4.8
(1.7/91.8)
7.0
(2.1/52.1)
21.1
(3.5/112.1)
10.5
(2.1/52.1)
18.7
(3.5/112.1)
VEGF_PWM
13.7
(3.4/66.9)
5.5
(1.5/94.4)
14.4
(3.4/66.9)
5.1
(1.5/94.4)
17.1
(0.2/83.4)
4.9
(0.1/33.1)
11.0
(0.2/83.4)
11.9
(0.1/33.1)
VEGF_P + C
21.7
(4.7/96.1)
9.4
(1.6/52.6)
27.2
(4.7/96.1)
9.3
(1.6/52.6)
12.3
(0/20.6)
5.2
(0.2/17.2)
9.6
(0/20.6)
5.2
(0.2/17.2)
VEGF_HSP70
32.8
(5.8/101.1)
10.6
(1.1/45.3)
35.5
(5.8/101.1)
16.0
(1.1/45.3)
54.0
(3/121.7)
14.4
(1.5/91.9)
42.7
(3/121.7)
15.7
(1.5/91.9)
VEGF_Pg
17.9
(0.5/48.1)
9.4
(0.8/63.6)
17.4
(0.5/48.1)
15.3
(0.8/63.6)
2.9
(0.3/33.7)
5.5
(0.1/30)
5.1
(0.3/33.7)
2.1
(0.1/30)
VEGF_Aa
27.4
(8.8/119.1)
27.7
(3.5/57.8)
27.0
(8.8/119.1)
29.5
(3.5/57.8)
47.8
(4.1/73.7)
27.8
(7.6/36.8)
23.9
(4.1/73.7)
30.4
(7.6/36.8)
VEGF_Tf
18.4
(4.1/89.4)
17.0
(4/90.1)
17.2
(4.1/89.4)
19.0
(4/90.1)
18.9
(3.6/126.8)
11.1
(0.1/27.3)
10.6
(3/126.8)
13.9
(0.1/27.3)
VEGF_PI
30.2
(7.1/94.4)
15.2
(4.6/72.8)
29.1
(7.1/94.4)
22.6
(4.6/72.8)
30.2
(20/97.8)
15.2
(0.1/49.9)
29.4
(10/97.8)
15.2
(0.1/49.9)
VEGF_unstim.
24.9
(0.9/91)
22.8
(4.5/135.5)
29.6
(0.9/91)
21.9
(4.5/135.5)
39.8
(15.3/79.4)
10.2
(6.4/42.4)
35.6
(9.5/79.4)
10.2
(6.4/42.4)
*
, #, and † denote significantly higher levels of cytokines for , , and (resp.), how they were found by Kruskal-Wallis tests in application to couple locus in table header (the minor homozygous locus was connected to a heterogeneous locus for the formal increase the statistical significance of results).
