(a) OVA and LPS-induced murine model of neutrophilic asthma. Mice were intranasally sensitized with OVA plus LPS on days 0, 1, 2, and 7 and then challenged with OVA on days 14, 15, 21, and 22. Anti-HMGB1 IgG or control IgG was intranasally administered a half an hour before each OVA challenge. One day after the final challenge, the mice were euthanized for further analyses. (b) A murine model of asthma by the transfer of DCs. Mice were intratracheally sensitized with PBS/DCs, HMGB1/OVA-DCs, or HMGB1 + anti-HMGB1/OVA-DCs on day 0 and then challenged with OVA on days 10, 11, and 12. One day after the final challenge, the mice were euthanized for further analyses.
