Relationship of MMP-14 and TIMP-3 Expression with Macrophage Activation and Human Atherosclerotic Plaque Vulnerability

Table 2

Primers for quantitative RT-PCR.

Primer		Sequence	Annealing temp. (°C)	Fragment size (bp)
MMP-14	Forward	GGAGACAAGCATTGGGTGTT	60	343
	Reverse	GGTAGCCCGGTTCTACCTTC
TIMP-3	Forward	CTTCCGAGAGTCTCTGTGGCCTTA	60	230
	Reverse	CTCGTTCTTGGAAGTCACAAAGCA
COX-2	Forward	GCCATGGGGTGGACTTAAATCATA	60	168
	Reverse	CAGGGACTTGAGGAGGGTAGATCA
CD206	Forward	CGGTGACCTCACAAGTATCCACAC	58	216
	Reverse	TTCATCACCACACAATCCTCCTGT
IkB	Forward	CTACTGGACGACCGCCACGACAGC	60	58
	Reverse	CGAGGCGGATCTCCTGCAGCTCCTTG
36B4	Forward	GCCAGCGAAGCCACGCTGCTGAAC	60	76
	Reverse	CGAACACCTGCTGGATGACCAGCCC