Chrysophanol Inhibits NALP3 Inflammasome Activation and Ameliorates Cerebral Ischemia/Reperfusion in Mice

Figure 3

Cellular distribution of NALP3 inflammasome components and IL-1β in the ischemic penumbra zone of cerebral cortex. Sections were triple-stained for NALP3, ASC, caspase-1 and IL-1β (red), NeuN (green), and nucleus (blue). In tMCAO group, the neuronal karyopyknosis and chromatin margination could be observed and there was a small amount of neutrophils in marginal zone of infarction. In the normal brain (Sham), caspase-1 and NALP3 were mainly present in the nucleus of neurons whereas IL-1β was mainly localized in the cytoplasm. ASC was present in both the nucleus and cytoplasm. After tMCAO, NALP3 and caspase-1 were redistributed predominantly to the cytoplasm, while ASC and IL-1β location remained unchanged.